AccuSomatic Amplification for Single Cell Sequencing enables the discovery of true somatic single nucleotide variations (SNVs) in single cells that could not be accurately detected by any prior method. Existing methods suffer from multiple sources of artifacts resulting in more than 20,000 false somatic SNV calls per cell and >90% false positive results. AccuSomatic Amplification eliminates >99% of errors in somatic SNV calls while maintaining the same detection sensitivity. Our service based on this technological breakthrough enables, for the first time, accurate detection of somatic SNVs unique to one cell, and allows researchers to unlock the full potential of single cell sequencing in disease research and drug development.
Single cell whole genome bisulfite sequencing (WGBS), a developing field first reported in 2015, allows the detection of DNA methylation from a single cell. SingulOmics is the only commercial service provider for single cell WGBS service, and our protocol has achieved top quality results, enabling epigenetic studies at the single cell level. Our protocol has been applied to >200 single cells, covering > 1 million C’s on CpG sites per cell with >0.99 bisulfite conversion rate.
Single cell RNA-Seq opens a new field in biology by allowing the study of cell-to-cell transcriptome heterogeneity. SingulOmics is highly experienced in single cell RNA sequencing and data analysis. We provide high-throughput single cell transcriptome profiling service using 10x Genomics Chromium Platform to examine thousands of cells per sample by cell-by-cell 3’ end counting of mRNA transcripts. To enable the discovery of transcriptomes and gene expressions of single cells at a deeper level, SingulOmics also offers deep single cell RNA-Seq service of individual cells. The same technology can also be applied to samples with limited number of cells or with ultra-low amount of input RNA.
News & Events
The Singles Seen: Sequencing Gets Specific - Science, November 2017
Einstein Researchers Publish Single-Cell Sequencing Prep Method, Launch Firm to Provide Service - Genomeweb, April 2017
Tool Can Accurately Detect Single Nucleotide Variations in Individual Cells - Labroots, March 2017