AccuSomatic Amplification for Single Cell Sequencing enables the discovery of true somatic single nucleotide variations (SNVs) in single cells that could not be accurately detected by any prior method. Existing methods suffer from multiple sources of artifacts resulting in more than 20,000 false somatic SNV calls per cell and >90% false positive results. AccuSomatic Amplification eliminates >99% of errors in somatic SNV calls while maintaining the same detection sensitivity. Our service based on this technological breakthrough enables, for the first time, accurate detection of somatic SNVs unique to one cell, and allows researchers to unlock the full potential of single cell sequencing in cancer, drug development, and aging research.
Single cell whole genome bisulfite sequencing (WGBS), a developing field first reported in 2015, allows the detection of DNA methylation from a single cell. SingulOmics is the only commercial service provider for single cell WGBS service, and our protocol has achieved top quality results, enabling epigenetic studies at the single cell level. Our protocol has been applied to >100 single cells, covering > 1 million C’s on CpG sites per cell with >0.99 bisulfite conversion rate.
Einstein Researchers Publish Single-Cell Sequencing Prep Method, Launch Firm to Provide Service - Genomeweb, April 21, 2017
Tool can Accurately Detect Single Nucleotide Variations in Individual Cells - Labroots, March 20, 2017