Single Cell DNA Sequencing & AccuSomatic Amplification
AccuSomatic Amplification for Single Cell Sequencing enables the discovery of true somatic single nucleotide variations (SNVs) in single cells that could not be accurately detected by any prior method. Existing methods suffer from multiple sources of artifacts resulting in more than 20,000 false somatic SNV calls per cell and >90% false positive results. AccuSomatic Amplification eliminates >99% of errors in somatic SNV calls while maintaining the same detection sensitivity. Our unique service enables researchers, for the first time, to unlock the full potential of single cell sequencing in disease research and drug development.
Single Cell RNA-Seq
Single cell RNA-Seq enables the study of cell-to-cell transcriptome heterogeneity, opening a new field in biology. SingulOmics is highly experienced in single cell RNA sequencing and data analysis. We perform high-throughput single cell transcriptome profiling on the 10x Genomics Chromium Platform through cell-by-cell 3’ end counting of mRNA transcripts. Our workflow incorporates dead cell removal or nuclei isolation depending on sample type and quality to ensure accurate results. To enable the discovery of single cell transcriptomes at a deeper level, SingulOmics also offers deep single cell RNA-Seq of individual cells. This technology can also be applied to samples with a limited number of cells or with ultra-low amounts of input RNA.
Single Cell ATAC-Seq
Single-cell ATAC Sequencing is a technique used to determine genome-wide chromatin accessibility at the single-cell level. With single-cell ATAC-Seq, chromatin profiling of large numbers of nuclei can be performed in parallel, resulting in fast, accurate epigenomic profiling. Using the industry-leading Chromium Single-Cell ATAC Solution from 10x Genomics, SingulOmics’ PhD-level scientists and bioinformaticians can analyze thousands of nuclei per run at single-cell resolution. In combination with Single-Cell RNA-Seq, our end-to-end services and integrated analysis enable researchers to link input regulatory signals with their output gene expression at the single-cell level.
Single Cell CNV
Analyzing copy number variation (CNV) at the single-cell level allows for cellular heterogeneity and clonal structure to be determined at high scale and resolution. As a pioneer in the field of single-cell analysis, SingulOmics has a highly experienced team who can lend their expertise to your project to ensure high-quality results. We utilize the cutting-edge 10x Genomics Chromium platform to provide Single-Cell CNV analysis. Profile hundreds to thousands of cells per sample and detect CNVs at 2 Mb resolution. SingulOmics offers a comprehensive solution, from amplification to sequencing to data analysis and visualization.
Single Cell Whole Genome Bisulfite Sequencing
Single cell whole genome bisulfite sequencing (WGBS), a developing field first reported in 2015, allows the detection of DNA methylation from a single cell. SingulOmics is the only commercial service provider for single cell WGBS service, and our protocol has achieved top quality results, enabling epigenetic studies at the single cell level. Our protocol has been applied to >200 single cells, covering > 1 million C’s on CpG sites per cell with >0.99 bisulfite conversion rate.
SingulOmics in the Media
The Singles Seen: Sequencing Gets Specific - Science, November 2017
Einstein Researchers Publish Single-Cell Sequencing Prep Method, Launch Firm to Provide Service - Genomeweb, April 2017
Tool Can Accurately Detect Single Nucleotide Variations in Individual Cells - Labroots, March 2017