single cell RNA sequencing imageHigh-Throughput or Deep Single Cell RNA-Seq Service

Single cell RNA-Seq opens a whole new field in biology by allowing the study of cell-to-cell transcriptome heterogeneity. It enables the discovery of cellular differences typically masked by standard, bulk RNA sequencing. Building on the unmatched expertise in single-cell genomics, transcriptomics and epigenomics developed since mid-2000s, SingulOmics is highly experienced in single cell RNA sequencing and data analysis.

We provide high-throughput single cell gene expression profiling service using 10x Genomics Chromium Single Cell Platform to examine thousands of cells per sample by cell-by-cell 3’ end counting of mRNA transcripts. Our end-to-end service solution enables identification of rare cell types, analysis of cellular heterogeneity, and understanding of individual cell states and cellular responses to environmental signals and conditions.

In addition, we provide 10x Genomics Chromium Single Cell Immune Profiling service. From the same input sample, we generate two or three libraries for (a) 5’ gene expression analysis, (b) T cell receptor repertoire profiling, and (c) B cell immunoglobulin repertoire profiling. These libraries are sequenced and analyzed integratively in downstream informatics analysis.

To enable the discovery of transcriptomes and gene expressions of single cells at a deeper level, SingulOmics offers deep single cell RNA-Seq service. The same technology can also be applied to samples with limited number of cells (1-1000 cells) or with ultra-low amount of input RNA (ultra-low-input RNA-Seq).

 

Service Workflow

High-Throughput 10x Genomics Single Cell RNA-Seq

10X Genomics Chromium Single Cell RNA sequencing workflow

 

Ultra-Low-Input / Deep Single Cell RNA-Seq

single cell RNA-Seq and ultra low input RNA-Seq workflow

Sample Requirements

High-Throughput 10x Genomics Single Cell RNA-Seq

Fresh cells are recommended for this procedure, but frozen cells can also be used as starting material.

(1) We recommend freezing 5x10^5 to 1x10^6 cells in 1 ml of freezing media per cryotube.

(2) If the number of cells is not a limiting factor, freeze 2x10^6 cells in 1 ml of freezing media per cryotube and prepare two (2) cryotubes per sample.

(3) Use standard freezing media (e.g. cell growth media + 20% FBS + 10% DMSO; or 90% FBS + 10% DMSO) and place the samples at -80°C while slowly decreasing the temperature.

Please click here for generic sample preparation guide and contact us for project-specific requirements.

 

Ultra-Low-Input / Deep Single Cell RNA-Seq

Samples can be submitted as (1) single cells (nuclei), or (2) frozen cells in cell suspensions, or (3) total RNA.

(1) Single cells (nuclei): Please click here to access instructions on how to collect single cells.

(2) Frozen cells of single population in cell suspensions: We provide single cell isolation service using CellRaftTM. Please click here for sample requirements.

If you have additional requirements for single cell isolation (e.g. single cell population from blood or tissues), please contact us.

(3) Total RNA, please click here for sample requirements.

Next Steps...

Contact us to learn more or get a price quote.