FocusSeq Technology – Cost-effective, Focused Sequencing of Genomic Regions of Interest

Our FocusSeq service is the ideal solution for studying variations in specific genes or other genomic areas of interest. FocusSeq technology is based on the design of targeted next-generation sequencing panels composed of hundreds of PCR targets. The resulting amplicons target specific genomic regions of interest, allowing detection of SNPs and indels faster and more cost-effectively than with whole-genome approaches. FocusSeq yields high-confidence results while also reducing the background noise typical of WGS and other higher throughput methods.

Our service includes the design, optimization, validation, and data analysis of custom panels, incorporating a highly multiplexed PCR-based workflow and amplicon sequencing. Our solution offers high coverage and redundancy, as well as high sensitivity and specificity, with the ability to analyze a few to hundreds of genetic targets. With our optimized protocols for biological samples such as blood, saliva, and frozen tissues, we generate high-confidence data for many areas of application, including cancer and genetic diseases.

At SingulOmics, our custom-designed PCR panels, in combination with our amplicon sequencing and data analysis, have greater proven efficacy in variant detection than conventional sequencing methods. We design targeted panels with high redundancy for each locus of interest, allowing the optimal representation of each region. By focusing on specific genomic areas, our technology can be particularly helpful for detecting low-frequency variants, which can be lost in whole-genome analysis. The FocusSeq solution can be applied to a wide array of samples, including blood, saliva, cell lines, and formalin-fixed paraffin-embedded (FFPE) or frozen tissues. Our methodology ensures highly reliable results and allows us to customize conditions for the specific requirements of each project, from the initial design to final data analysis.