End-to-End Bulk RNA-Seq Service

RNA sequencing (RNA-Seq) is a powerful technique for analyzing the transcriptome—the complete set of RNA transcripts produced by the genome under specific conditions. By accurately quantifying gene expression and identifying novel transcripts, isoforms, and gene fusion events, RNA-Seq provides a high-resolution view of cellular mechanisms. Bulk RNA sequencing is particularly effective for studying differential gene expression in various contexts, such as disease states or treatment responses.

At Singulomics, we provide a complete bulk RNA sequencing service that delivers accurate, high-quality data, even with challenging samples like small tissue specimens or limited cell numbers. Our service is tailored to meet your specific research needs, ensuring you receive reliable and insightful gene expression data.

Our service includes:

Total RNA Isolation (Optional): Efficient isolation of RNA from various sample types, including fresh-frozen or FFPE tissue, as well as from limited numbers of cells.
RNA Quality Assessment: Rigorous assessment of RNA quality and integrity to optimize sequencing results, ensuring valuable data is obtained.
RNA Enrichment: Offering both Poly(A)+ RNA enrichment or rRNA depletion, focusing on the most relevant data for your study.
Library Preparation and QC: High-quality library preparation for next-generation sequencing, ensuring data integrity throughout the process.
Next-Generation Sequencing: Conducted on the Illumina platform with 2 x 150 bp reads, delivering high-quality data with a Q30 score of ≥ 85%.
Comprehensive Bioinformatics Analysis: In-depth analysis including gene expression quantification, functional enrichment analysis, and more, ensuring your data is ready for publication.

Singulomics is dedicated to supporting your research with precise, high-quality mRNA-Seq solutions that yield actionable insights, empowering your discoveries.

Service Workflow

Sample Requirements

We accept a wide range of sample types for RNA sequencing, including total RNA, fresh frozen tissue, preserved tissues such as FFPE, blood samples, cultured cells, and more. Whether you’re working with complex clinical samples, small tissue specimens, or specific cell populations, our service is equipped to handle and process diverse sample types to meet your research needs. Please contact us to discuss the specific requirements for your sample type.

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